Signal Transduction Reagents and Kits
Promega GSH/GSSG-Glo™ Glutathione Assay
The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells.
Promega ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.
Promega NAD/NADH-Glo™ Assay
The NAD/NADH-Glo™ Assay is a bioluminescent, homogeneous single-reagent-addition assay for detecting total oxidized and reduced nicotinamide adenine dinucleotides and determining their ratio in biological samples or in defined enzyme reactions.
Promega P450-Glo™ CYP3A4 Assay with Luciferin-IPA
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
Promega P450-Glo™ CYP1A2 Induction/Inhibition Assay
The P450-Glo™ CYP1A2 Assay and Screening Systems provide a complete set of reagents for performing luminescent cytochrome P450 assays
Promega ADP-Glo™ Kinase Assay+ MAPKAPK2 Kinase Enzyme System
Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase that is regulated via direct phosphorylation by p38 MAP kinase.
Promega ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System
Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ∼460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.
Promega Kinase-Glo™ Max Luminescent Kinase Assay
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Promega Glutamine/Glutamate-Glo Assay, 50ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega NADPH Regeneration System
The NADPH Regeneration System is an enzyme substrate combination that reduces NADP+ to NADPH.
Promega P450-Glo™ CYP3A4 Assay (Luciferin-PPXE) DMSO-Tolerant Assay
The P450-Glo CYP3A4 Assay System provides a homogeneous, luminescent method for measuring CYP3A4 activity and the screening system contains a complete set of reagents for performing the assay.
Promega ENLITEN™ ATP Assay System
The ENLITEN ATP Assay System is used to measure ATP levels for the indirect detection of biocontamination.
Promega ALK6 Kinase Enzyme System, 1mg
ALK6 (also known as BMPR1B) is a member of the transmembrane serine/threonine kinase that is the member of the bone morphogenetic protein (BMP) receptor, which is closely related to the activin receptors, ACVR1 and ACVR2.
Promega CDK3/CyclinE1 Kinase Enzyme System, 1mg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega CK1 alpha 1 Kinase Enzyme System, 1mg
Full-length recombinant human CK1 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1 alpha 1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes.